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KMID : 0361319790110010003
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Korean Journal of Medical Technologists
1979 Volume.11 No. 1 p.3 ~ p.12
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Comparative study of Malloy-Evelyn and Jendrassik-Grof methods on serum bilirubin measurement
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Abstract
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Malloy-Evelyn method is simple and widely used, but turbidity and faintness of the color intensity n ay interfere correct measurement of the normal and direct bilirubin values. The disadvantages also include greater interference by hemolysis and foam formation which may prolong reading time and cause error of the value.
A comparative experiment between Malloy-Evelyn and Jendrassik-Grof methods: using caffeine as an accelerator and utilizing better color reaction in strong alkali in the latter. The following conclusions are made.
1. There is a significant difference of total bilirubin value between two methods; J-G method showed superior reproducibility.
2. J-G moethod revealed no turbidity and foam formation. It also requires shorter testing time and easier manipulation compare to that of M-E method.
3. Both methods were greatly influenced by hemolysis, showing lower values but better result was encountered by adding ascorbic acid to J-G method.
4. Measuring direct bilirubin in J-G method, the color intensity continuously increases by significant degree for several minutes if reducing agent is not added as in M-E metod.
5. Reconsideration of the 1 minute value of direct bilirubin measurement is stresesd.
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